Synthetic peptide corresponding to the C-terminal sequence of propeptide form of VEGF-C.
Vascular endothelial growth factors (VEGF) are structurally and functionally related growth factors, that may play important roles in the formation of vascular systems during embryonic development, in regulation of capillary growth in adults, and in maintenance of normal vasculature. VEGF-C is a ligand for receptor tyrosine kinase VEGFR-3 (Flt-4), which is mainly expressed in the endothelium of lymphatic vessels. VEGF-C mRNA has been detected in human heart, lung, muscle, ovary, placenta, and small intestine. It is also detected in many malignant tumors, including breast carcinomas, squamous cell carcinomas, lymphomas, melanomas, sarcomas and ademomas. VEGF-C is synthesized as a precursor protein called prepropeptide, which undergoes a series of proteolytic steps to give the mature form of VEGFC. The prepropeptide is cleaved and forms a homo-dimer between two identical propeptides. This dimer is then processed to form a secretable tetramer that consists of two 29kD and two 31kD peptides, which is then secreted extracellularly and processed to produce the mature form that consists of two 21kD peptides. Studies suggest the 29/31kD form is the most prevalent form of VEGF-C in many biological systems. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Immunogen): 0.1-1ug/ml Western Blot (Human): 2-5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: U937 lymphoma cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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