Verotoxin I (SLT-1b, STX-1b) (Biotin), IgG1, Clone: [5E455], Mouse, Monoclonal

Product Overview

• Article Name: Verotoxin I (SLT-1b, STX-1b) (Biotin), IgG1, Clone: [5E455], Mouse, Monoclonal
• Biozol Catalog Number: USB-V2121-60A-BIOTIN
• Supplier Catalog Number: V2121-60A-Biotin
• Alternative Catalog Number: USB-V2121-60A-BIOTIN-100
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: ELISA
• Immunogen: Purified toxoid

Product Description

Shiga like toxins, also called verotoxins, inhibit protein synthesis in eukaryotic cells. These toxins play a role in hemorrhagic colitis and hemolytic uremic syndrome. They damage endothelial cells in both kidney and brain, causing renal failure and neurological complications. The protein structure consists of two domains: the A polypeptide confers the activity, and the B polypeptide pentamer confers the receptor-specific binding. The A polypeptide has an N-glycosidase activity that cleaves an adenine from the 28S rRNA of the 60S cytoplasmic ribosome. This activity renders the 28S rRNA unable to interact with the elongation factors EF-1 and EF-2. Thus inhibiting protein synthesis. The B polypeptide forms a pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3). Shiga like toxins then enter the cells by receptor-mediated endocytosis. The effects of shiga like toxin 1 and shiga like toxin 2 have been shown to vary with cell type because Gb3 receptors are present in certain tissues, particularly sensory neurons and renal cells. In those cell types, the receptor concentration and binding is modulated by factors such as tumor necrosis factor (TNF), interleukins, and fatty acid content of the membranes. Shiga like toxin 1 and shiga like toxin 2 differ in their effects on various tissues. They have both been shown to induce apoptosis in several different cell types. Once the shiga like toxins have been endocytosed, they are retrogradely transported through the golgi apparatus to the rough endoplasmic reticulum where they effectively target the ribosomes. In addition to inhibiting protein synthesis, shiga like toxins induce cytokines such as interleukin-1, interleukin-6, and interleukin-8. Shiga like toxins are very toxic and should be handled with extreme caution. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [5E455]
• Isotype: IgG1
• Purity: Purified by Protein A affinity chromatography
• Form: Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.