Verotoxin II A Subunit (Shiga-like Toxin 2 Subunit A, SLT-2a, SLT-IIa), IgG1, Clone: [9G301 11E10], Mouse, Monoclonal
Biozol Catalog Number:
USB-V2121-60C
Supplier Catalog Number:
V2121-60C
Alternative Catalog Number:
USB-V2121-60C-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, WB
Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis. Applications: Suitable for use in Western Blot, ELISA and Functional Assays. Other applications not tested. Recommended Dilutions: Western Blot: 1:10 ELISA: 1:10 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[9G301 11E10]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography. Endotoxin: 0.5mg/ml
Form:
Supplied as a liquid in PBS, 0.1% BSA
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