Synthetic peptide corresponding to the C-terminus of human villin 1. Species Sequence Homology: rat, 100%.
Villin is a member of the gelsolin family of calcium-regulated actin-binding proteins. Unlike the ubiquitously expressed gelsolin, villin expression is restricted to simple epithelia of the gastrointestinal and urogenital tracts. It is localized to the apical cytoplasm and brush borders of these cells. Villin functions in the regulation of actin dynamics in the apical epithelium, capping, nucleating and/or severing actin filaments in a calcium-dependent manner and regulating cell shape in response to external stimuli. Phosphorylation of villin at Tyr60, 81 and 256 may be involved in the regulation of cell migration. Expression of villin is increased in colorectal cancers, and villin 1 function appears to be involved in progressive cholestasis and hepatic failure. Applications: Suitable for use in Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence (IC): 1:100 Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
