Purified Vimentin from porcine eye lens. Cellular Localization: Cytoplasmic
Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin/paraffin): 1:50 for 20 minute at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes. followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Sarcomas Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[0.N.602]
Purity:
Purified by Protein G affinity chromatography from ascites.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
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