Purified recombinant vimentin (Vm) produced in bacteria. This protein constitutes a 20kD fragment from the amino-terminus of vimentin.
Applications: Suitable for use as an enzyme labeled primary antibody in Immunoelectrophoresis (IEP) and ELISA applications. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied in a buffered stabilizer solution containing 50% glycerol. Labeled with Horseradish peroxidase (HRP) by the periodate method. Rz Ratio (Reinheitszahl, A403/A280): 0.46
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