Vinculin (VIN-1), IgG1, Clone: [0.T.153], Mouse, Monoclonal
Catalog Number:
USB-V2122-50
- Images (0)
| Article Name: | Vinculin (VIN-1), IgG1, Clone: [0.T.153], Mouse, Monoclonal |
| Biozol Catalog Number: | USB-V2122-50 |
| Supplier Catalog Number: | V2122-50 |
| Alternative Catalog Number: | USB-V2122-50-200 |
| Manufacturer: | US Biological |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IHC, IP, WB |
| Immunogen: | Human vinculin. |
| Vinculin, a cytoskeletal protein found in adherens type cell-cell and cell-extracellular matrix junctions, displays considerable heterogeneity. At least three 117kD and two 130kD isoforms (muscle specific metavinculin) have been detected. Vinculin binds talin and a-actinin in adhesion plaques and may play either a structural or a regulatory role in these structures. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects Vinculin in RIPA lysates of human A431 cells, previous lots detected Vinculin in RIPA lysates from chicken embryonic fibroblasts (CEF) and mouse 3T3 fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2122-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10ug/ml will stain Vinculin in Ref-52 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Optimal dilutions to be determined by the researcher. Positive Antigen Control (Included): A0001-50, Non-stimulated A431 cell lysate. Use 20ug per lane for minigels. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Product Guarantee and Expert Support
