13 amino acid peptide from near the amino terminus of human VISA.
Two distinct signaling pathways activate the host innate immunity against viral infection. One pathway is reliant on members of the Toll-like receptor (TLR) family while the other uses the RNA helicase RIG-I as a receptor for intracellular viral double-stranded RNA as a trigger for the immune response (1). VISA is a mitochondrial membrane protein that was identified as a critical component in the IFN-beta signaling pathways that recruits IRF-3 to RIG-I, leading to its activation and that of NF-kappaB (2). VISA is also thought to interact with other components of the innate immune pathway such as the TLR adapter protein TRIF, TRAF2 and TRAF6. VISA also interacts with the IKKalpha, IKKbeta and IKKepsilon kinases through its C-terminal region. Cleavage of this region by the Hepatitis C virus (HCV) protease allows HCV to escape the host immune system (3). At least three isoforms of VISA are known to exist. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml. See V2124-20D for control peptide. Optimal dilutions to be determined by the researcher. Positive Control: A20 cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
