Recombinant protein corresponding to 191aa from human Visinin-like protein 1 and 10 extra aa, N-terminal His-tag expressed in E. coli (P62760).
Visinin-like protein 1 (VILIP-1) is a cytoplasmic protein of low molecular weight (~22kD) consisting of 191aa residues. It belongs to the visinin/recoverin subfamily of neuronal calcium sensor proteins involved in calcium-dependent signal transduction mechanisms in neurons. It is found primarily in the brain and nerve cells, but it also has a peripheral distribution in liver, lung, kidney, spleen, pancreas and colon. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MKHHHHHHAS MGKQNSKLAP EVMEDLVKST EFNEHELKQW YKGFLKDCPS GRLNLEEFQQ LYVKFFPYGD ASKFAQHAFR TFDKNGDGTI DFREFICALS ITSRGSFEQK LNWAFNMYDL DGDGKITRVE MLEIIEAIYK MVGTVIMMKM NEDGLTPEQR VDKIFSKMDK NKDDQITLDE FKEAAKSDPS IVLLLQCDIQ K Storage and Stabillty: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O. Allow the pellet to dissolve completely.
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