Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin), Sheep
Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin), Sheep
Vitamin D Binding Protein (VDBP, VDB, DBP, DBP/GC, Gc Globulin, Group Specific Component, Group-specific Component, GC, hDBP, Vitamin D Binding alpha Globulin), Sheep
Biozol Catalog Number:
USB-V2130-12
Supplier Catalog Number:
V2130-12
Alternative Catalog Number:
USB-V2130-12-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Recombinant protein corresponding to Leu17-Ser476 from mouse Vitamin D Binding Protein, expressed in NS0 cells. Uniprot/Swiss Accession: P21614
VDBP (Vitamin D binding protein, also group-specific component and GC-globulin) is a 52-58kD, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26-476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346-421, a second shows a 67 aa substitution for aa 345-421, and a third shows a 34 aa substitution for aa 346-423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17-476) is 77% and 90% aa identical to human and rat VDBP, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml in lysates of mouse liver Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
