Synthetic peptide corresponding to 15aa (aa495-511) from the C-terminus of VSV, conjugated to KLH
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag- epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins. Applications: Suitable for use in Immunofluorescence. Other applications not tested. Recommended Dilutions: Immunofluorescence: 1:2000-1:10,000 Optimal dilutions to be determined by the researcher. Absorption: 552nm Emission: 570nm Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Cy3 conjugates are sensitive to light.
Clonality:
Monoclonal
Clone Designation:
[11C165]
Purity:
Purified by affinity chromatography
Form:
Supplied as a lyophilized powder from PBS, 1% BSA, 0.05% sodium azide. Labeled with Cy3. Reconstitute with 100ul sterile PBS.
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