VSV-G Epitope Tag (Vesicular Stomatitis Virus Glycoprotein), Rabbit
Biozol Catalog Number:
USB-V5000-01H
Supplier Catalog Number:
V5000-01H
Alternative Catalog Number:
USB-V5000-01H-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IF, WB
Immunogen:
Synthetic peptide corresponding to aa501-511 of vesicular stomatitis virus glycoprotein (VSV-G).
VSV is a member of the Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G). G is a type I membrane glycoprotein and is responsible for viral binding to cell surface receptors and initiating the infectious process. VSV-G or vesicular stomatitis virus glycoprotein is found within the pseudo lentiviral cloning vector pHCMV-VSV-G. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:8000-1:32,000 Immunofluorescence: use paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1:200. At higher concentrations, a filamentous background staining may be present. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
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