Mouse anti-bovine WC4 antibody, clone CC55 recognizes the bovine WC4 cell surface antigen, a ~90kDa molecule expressed by a subpopulation of B cells in peripheral blood and lymphoid tissues (Howard et al. 1993). It is suggested that WC4 like SWC7 in pigs is the bovine orthologue of human CD19 (Naessens and Howard 1991, Ikebuchi et al. 2013). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100, 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[CC55]
Isotype:
IgG1
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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