Bovine WC1 is a 215/300kD antigen expressed on the majority of gamma/delta T lymphocytes. These cells also express low levels of CD5, but are negative for other B and T cell markers. The WC1 antigen is reported to be involved in a number of processes, including activation of gamma/delta T cells and the development of a Th1-biased acquired immune response. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10, 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[10F583]
Isotype:
IgG2a
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
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