Wisteria floribunda (WFA, Japanese Wisteria) (Biotin)
Biozol Catalog Number:
USB-W1500-06A
Supplier Catalog Number:
W1500-06A
Alternative Catalog Number:
USB-W1500-06A-1,USB-W1500-06A-2
Manufacturer:
US Biological
Category:
Molekularbiologie
Purified Wisteria floribunda lectin (WFA), Biotin conjugated. Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose. Carbohydrate Specificity: N-Acetylgalactosamine Inhibitory Carbohydrate: N-Acetylgalactosamine >> Lactose >> Galactose Activity: 15-30ug/ml will agglutinate type A1 human erythrocytes. Less than 0.5ug/ml will agglutinate neuraminidase treated erythrocytes. Chemical Used for Conjugation: Biotinyl N-hydroxysuccinimide ester (BNOHSE) Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a lyophilized powder from PBS, pH 7.3. Labeled with Biotin. Reconstitute with 1ml sterile PBS.
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