Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (HRP), Rabbit
Catalog Number:
USB-W3011-02B-HRP
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| Article Name: | Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) (HRP), Rabbit |
| Biozol Catalog Number: | USB-W3011-02B-HRP |
| Supplier Catalog Number: | W3011-02B-HRP |
| Alternative Catalog Number: | USB-W3011-02B-HRP-100 |
| Manufacturer: | US Biological |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IHC, WB |
| Immunogen: | Synthetic peptide corresponding to the central region of human, mouse and rat Wnt3A (KLH). |
| The Wnt family of consists of ligands involved in cell development, fate determination, organogenesis and oncogenesis. Information on Wnt signaling has been inferred from genetic analysis of wingless (wg) signaling in Drosophila. The Wnt signaling cascade initiates at the cell membrane when Wnt interacts with its receptor, a member of the Frizzled (Fz) transmembrane receptor family and lipoprotein receptor-related proteins 5 and 6 (LRP-5/6). This interaction relays the signal into the interior of the cell where disheveled (dsh) is brought to the membrane and gets phosphorylated/activated. Dsh promotes the inactivation of GSK3 kinase activity, thus preventing the phosphorylation of beta-Catenin and allowing it to accumulate as it avoids ubiquination and subsequent proteosome degradation. Stable beta-Catenin accumulates in the cytoplasm where it interacts with lymphoid enhancer factor/T cell factor (LEF/TCF) and translocates into the nucleus. This results in activation of TCF-responsive genes which are thought to play key roles in development and cancer. Negative regulators of the pathway may include axin and the tumor suppressor APC. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Paraffin Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody. |
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