Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5), IgG2a, Clone: [11C65], Rat, Monoclonal
Biozol Catalog Number:
USB-W3028-01
Supplier Catalog Number:
W3028-01
Alternative Catalog Number:
USB-W3028-01-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Gln38-Lys380 of mouse Wnt-5a expressed in CHO cells.
Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 8-25ug/ml Immersion fixed frozen mouse embryo tissue sections. Western Blot: HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c) tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
