Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6), Sheep
Biozol Catalog Number:
USB-W3036-01
Supplier Catalog Number:
W3036-01
Alternative Catalog Number:
USB-W3036-01-100
Manufacturer:
US Biological
Host:
Sheep
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
E. coli-derived recombinant human Wnt-6 peptide containing rhWnt-6 (aa35-75 and 238-299) joined by a linker peptide, UniProt Q9Y6F9
Human Wnt-6 is a 46 kD, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse and canine Wnt-6 are identical. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml Immunohistochemistry: 5-15ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
