Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member), IgG2a, Clone: [7H100], Rat, Monoclonal
Biozol Catalog Number:
USB-W3037-10
Supplier Catalog Number:
W3037-10
Alternative Catalog Number:
USB-W3037-10-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
ELISA, IHC
Immunogen:
Recombinant protein corresponding to aa22-52 and aa195-269, connected by a Gly-Ser linker from mouse Wnt8b, expressed in E. coli (AAD31816). Species Sequence Homology: rat 100%, canine 98% and human 97%.
Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development. Applications: Suitable for use in Immunohistochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects Wnt-8b in immersion fixed frozen sections of embryonic mouse brain Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
