Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member), IgG2a, Clone: [7H99], Rat, Monoclonal
Biozol Catalog Number:
USB-W3037-11
Supplier Catalog Number:
W3037-11
Alternative Catalog Number:
USB-W3037-11-100
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Recombinant protein corresponding to Trp22-Glu52, Leu195-Arg269 from mouse Wnt-8b, expressed in E. coli.
Mouse Wnt-8b (Wingless related integration site family member 8b) is a 40-50 kD secreted glycoprotein member of the Wnt family of proteins that function as ligands for the Frizzled family of receptors. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 5ug/ml using lysates of KATO-III human gastric carcinoma cell line Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
