Wnt9a (Wingless-type MMTV (mouse mammary tumor virus) integration site family member 9a), IgG1, Clone: [8H145], Mouse, Monoclonal
Biozol Catalog Number:
USB-W3037-59
Supplier Catalog Number:
W3037-59
Alternative Catalog Number:
USB-W3037-59-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Recombinant protein corresponding to Pro48-Glu92 and Arg231-Phe304 from human Wnt9a, expressed in E. coli.
Wnt-9a (Wingless related integration site family member 9a, formerly Wnt-14) is a member of the Wnt family, which are ligands for frizzled family receptors. Wnt-9a is a secreted glycoprotein expressed in skeletal muscle and heart as well as in B-CLL and gastric, pancreatic and breast cancer cells. Human Wnt-9a is 98% aa identical with both mouse and rat Wnt-9a. Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug/10-6 cells MCF-7 human Breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin. Western Blot: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
