Indigo-blue chromogenic substrate used in the detection of beta-glucuronidase in bacterial colonies. The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the $-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants.2,3 X-Gluc has reported applications in the detection of contaminated food samples such as meat, dairy, and shellfish products.4,5 X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli.6 It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods. BENEFITS -Saves time compared to other E. coli detection methods... 24 hour direct plating method7,9,11 -Minimizes errors...less than 1% false negatives and less than 5% false positives7,8,9 -Easy visual d etection...8max is in visible range -Sensitive for p resence/ab sence of E. coli...eliminates limitation s of very rapid presence/absence or chemiluminometric methods12 Applications: Suitable for use in environmental testing of E. coli in potable water, detection of GUS expression in the study of trans cription, translation, and protein transport events, determination of urinary tract infections and identifying food products contaminated with E. coli. Solubility (1% H2O): Colorless, clear, complete Water (KF): 1% H-NMR: Conform to structure DNase or Protease: None Detected Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight:
444.7
Purity:
98% (HPLC)
Form:
White to off white crystalline powder
CAS Number:
[129541-41-9]
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