Zeranol is a synthetic zearalenone (6-reduced zearalenone) marketed as Ralgro used as a growth promotant in cattle. The use of implants of zeranol (alpha-zearalanol), a widely-used anabolic growth promoter, in food animals results in very low residues of this compound in the edible tissues. In comparison, residues of myco-oestrogens--specifically the beta-resorcylic acid lactones (RALs) such as zearalenone and its metabolites--commonly found as contaminants of cereals are much greater. Zeranol, the RALs and their metabolites are all closely related chemically, sterically and biologically. The biological effects of these compounds on reproduction, oestrogenicity, teratogenicity, genotoxicity and carcinogenicity are compared. No toxicological effects have been reported that cannot be attributed to the oestrogenic character of these molecules. In comparison with oestradiol, zeranol and the related myco-oestrogens are 100-1000 times less active hormonally. It is concluded that a likely no-observed-effect level (NOEL) for these compounds will be of the order of 0.05-0.1mg/kg body weight/day. This would result in a safety margin greater than 500-fold between the expected dietary exposure to these compounds (considered together) and the likely NOEL. On the basis of exposure to zeranol alone, this margin of safety would increase to more than 35,000. Applications: Suitable for use in ELISA. Other applications have not been tested. Recommended Dilutions: ELISA: 1:1200-1:1500 (competitive immunoassay) Optimal dilutions to be determined by the researcher. Matched Pairs: Capture: Z0405-03 Detection: Z0405-04 Competitive ELISA: Matching antibody Z0405-04 Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified
Form:
Supplied as a liquid in 20mM PBS, pH 7.2. Labeled with horseradish peroxidase (HRP). Do not add azide.
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