Zinc Transporter ZIP3, Control Peptide (Solute Carrier Family 39 Member 3, Zrt- and Irt-like Protein 3, ZIP-3, hZIP3, SLC39A3, ZIP3)
Biozol Catalog Number:
USB-Z0510-04A
Supplier Catalog Number:
Z0510-04A
Alternative Catalog Number:
USB-Z0510-04A-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control peptide for Z0510-04. Zinc is an essential nutrient for all organisms because of the many important roles this metal plays. Movement of zinc into and out of cells and subcellular organelles is mediated by zinc transporter proteins. In many organisms, zinc uptake is mediated by members of the ZIP family of metal ion transporters. In mammals, the Zip1, Zip2, Zip3, Zip4, Zip5, LIV- 1 (Zip6), KE4 (Zip7), and BIGM103 (Zip8) proteins have been implicated in zinc uptake in a variety of cell and tissue types. Zip3 (SLC39A3) encodes a 314aa protein that has an N-terminal signal peptide. Overexpression of the mZIP3 homolog in HEK293 cells increased Zn uptake in similar fashion to mZIP1 and mZIP2, however, mZIP3 specificity for zinc could be less than reported for the two other Zip transporters. Zinc-depleted THP1 cells have downregulated Zip3 expression. Source: Synthetic peptide corresponding to 17aa of mouse ZIP3 (Accession AAH05502), N-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
