ZBTB16 (Zinc Finger And BTB Domain-containing Protein 16, Promyelocytic Leukemia Zinc Finger Protein, Zinc Finger Protein 145, Zinc Finger Protein PLZF, PLZF, ZNF145), IgG2a, Clone: [11n205], Mouse, Monoclonal
Biozol Catalog Number:
USB-Z0744-35B
Supplier Catalog Number:
Z0744-35B
Alternative Catalog Number:
USB-Z0744-35B-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
FC, WB
Immunogen:
Recombinant protein corresponding to Met1-Gln254 from human PLZF expressed in E. coli.
Human PLZF is a 74kD nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673aa protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RARa and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96% and 96% aa identity with rat, mouse, canine, and bovine PLZF, respectively. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/106 cells Western Blot: 1ug/ml Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
