A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies from the N-terminal to the 80aa region (the alpha-motif) present in the a+isoform but absent in the alpha- isoform due to alternative splicing.
ZO-1 is a peripheral membrane protein that is associated with tight junctions (zonula occludentes) which are found on the cytoplasmic surface of epithelial and endothelial cell membranes. ZO-1 has a MW of 225kD and is found in two isoforms that differ by the presence or absence of an 80 amino acid region known as motif-a. These two isoforms are the basis for determining two classes of tight junctions. ZO-1 is known to interact with ZO-2. ZO-1 is homologous to dlg, the Drosophila tumor suppressor. Both are members of a putative signal transduction protein family whose members contain a SH3 domain and a domain homologous to the enzyme guanylate kinase. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Frozen, acetone fixed): 2-5ug/ml Immunohistochemistry (FFPE): 2-5ug/ml Protease XIV pretreatment is required Immunofluorescence (IC): 5-10ug/ml Optimal dilutions to be determined by the researcher. Positive Controls for Immunoprecipitation: Canine MDCK and human Caco-2 cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
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