Synthetic peptide corresponding to KWLDRYRQIRPCTS from the C-terminal of human ZRANB1. Species Sequence Homology: bovine, canine, mouse, porcine and rat
Positive regulator of the Wnt signaling pathway that specifically cleaves Lys-63-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. May also modulate TNF-alpha signaling. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:4000 Western Blot: 1-3ug/ml,using human brain (cerebellum) lysates. Band at ~80kD. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
