The strong cell wall of S. aureus Protein A strain provides an excellent anchor for active Protein A molecules. After fix and kill, the cells become fine solid particles with active Protein A molecules on it. The Protein A particles can be used in absorbance and purification of monoclonal and polyclonal antibodies, and immobilizing antibody in immunoprecipitation and co-immunoprecipitation assays. Appearance: Supplied as a lyophilized powder from PBS, pH 7.4, 0.05% thimerosal. Reconstitute with 20ml or 100ml sterile ddH2O. The product will yield a 10% suspension of fixed and killed S. aureus, Cowan I strain. Binding Capacity: One ml of the 10% suspension is capable of binding 2mg of rabbit IgG. Applications: Suitable for use in Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a lyophilized powder from PBS, pH 7.4, mannitol. Reconstitute with 20ml or 100ml sterile ddH2O. The product will yield a 10% suspension of fixed and killed S. aureus, Cowan I strain.
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