codon-optimized FLP (FLPo) with mCherry Adenovirus (Ad-mCherry-FLPo)

Product Overview

• Article Name: codon-optimized FLP (FLPo) with mCherry Adenovirus (Ad-mCherry-FLPo)
• Biozol Catalog Number: VEB-1778
• Supplier Catalog Number: 1778
• Alternative Catalog Number: VEB-1778
• Manufacturer: VectorBuilder
• Category: Molekularbiologie
• Alternative Names: codon-optimized FLP (FLPo) with mCherry Adenovirus

Product Description

FLP is a site-specific recombinases (SSR) from the i integrase family, which recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. FLPo is a mouse codon optimized FLPe that greatly increases the FRT recombination efficiency in mouse cells. This adenovirus express both FLPo and mCherry as a fluorescent marker. FLPo and mCherry are driven by the same CMV promoter and separated by a 2A peptide.

Product Properties

• Buffer: DMEM, 2% BSA, 2.5% Glycerol