5' EndTag™ DNA/RNA Labeling Kit, 1 Kit

Product Overview

• Article Name: 5' EndTag™ DNA/RNA Labeling Kit, 1 Kit
• Biozol Catalog Number: VEC-MB-9001
• Supplier Catalog Number: MB-9001
• Alternative Catalog Number: VEC-MB-9001
• Manufacturer: Vector Laboratories
• Category: Kits/Assays

Product Description

5 EndTag DNA/RNA Labeling Kit, 1 Kit

Additional Information

Description

End labeling is a favored method for applications in which an internal label might interfere with hybridization or sequence specific protein binding.

 

Features of the 5' EndTag DNA/RNA Labeling Kit:

• Enables simple and uniform labeling of the 5' end of a DNA, RNA or unmodified oligonucleotide molecule.
• Preserves the unmodified 3' end of the oligonucleotide (ideal for the labeling of PCR primers as a result).
• Compatible with a choice of diverse labels - fluorophores, biotin, haptens, or affinity ligands*

 

Specifications

• Unit Size: 1 kit
• Target for Labeling: DNA, Oligonucleotides, RNA
• Required Reactive Group: 5 '-OH group of nucleic acid
• Tag/Group Incorporated: Thiol

 

Kit Components

What's in the box?

Materials suitable to perform 10 end labeling reactions of up to 0.6 nmols of 5' ends (e.g. about 5 μg of a 25 base oligo) per reaction. This includes:

• T4 polynucleotide kinase
• 10x reaction buffer
• ATPγS
• Precipitant
• Alkaline Phosphatase

 

*Note: Thiol-reactive labeling reagent is not included in the kit and must be purchased separately. Labels suitable for this kit include:

• Biotin (Long Arm) Maleimide
• DNP Maleimide
• Fluorescein (Long Arm) Maleimide
• Fucose Maleimide
• Texas Red® Maleimide

Note regarding shipping: This item is shipped on wet ice and therefore requires additional shipping charges.

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