Vector Blue Substrate Kit, Alkaline Phosphatase (AP),

Catalog Number: VEC-SK-5300
Article Name: Vector Blue Substrate Kit, Alkaline Phosphatase (AP),
Biozol Catalog Number: VEC-SK-5300
Supplier Catalog Number: SK-5300
Alternative Catalog Number: VEC-SK-5300,SK-5300
Manufacturer: Vector Laboratories
Category: Kits/Assays
Application: IHC
Conjugation: AP
Vector Blue Substrate Kit, Alkaline Phosphatase (AP)



The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.




  • Greater sensitivity than conventional substrates
  • Consistent and reliable
  • Ideal for IHC/ICC/ISH and blots
  • Fluorescent
  • Heat Stable
  • Permanent mounting
  • Stock solutions supplied in convenient dropper bottles promoting ease of handling
  • No wait times for mixing and dissolving powders or tablets
  • Sufficient reagents to produce 200 ml of working solution
  • Excitation: broad, peak at ~500 nm*
  • Emission: broad, peak at ~680 nm*


*Histochem J. (1998) 8:577




More Information
Unit Size 1 kit
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Detection Enzyme(s) Alkaline Phosphatase
Substrate Reaction Product Precipitating Reaction Product
Chromogen Color Blue
Color of Fluorescence Far Red
Microscopy Brightfield, Fluorescence, Spectral Imaging
Additional Substrate Properties Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Mounting Aqueous (Hardening), Aqueous (Non-Hardening), Non-Aqueous (Permanent)
Safety Title WARNING: Reproductive Harm -


Kit Components


Kit Contents:


  • 4 ml Vector Blue Reagent 1
  • 4 ml Vector Blue Reagent 2
  • 3 ml Vector Blue Reagent 3
Vector® Blue Substrate Kit, Alkaline Phosphatase (AP) Immunohistochemical Staining