ELISA and Assay Development
Develop your own ELISA or Assay perfectly tailored to your needs
ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
ELISAs are typically performed in 96-well (or 384-well) polystyrene plates, which will passively bind antibodies and proteins. It is this binding and immobilization of reagents that makes ELISAs so easy to design and perform. Having the reactants of the ELISA immobilized to the microplate surface makes it easy to separate bound from non-bound material during the assay. This ability to wash away nonspecifically bound materials makes the ELISA a powerful tool for measuring specific analytes within a crude preparation.
Some of our partners list kits that are measuring activity of a specific enzyme/process, as “Assay Kits.” This is done to differentiate them in a way that could be helpful for people.
In this case in an ELISA the indicated protein is quantified and in an assay the indicated protein activity is quantified (or the indicated molecule is being measured by another non-antibody-based means)
At BIOZOL you have the opportunity to develop your own ELISA or assay together with our experienced partners.
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