Immunohistochemistry

Blocking of Endogenous Peroxidase Activity

Vector LaboratoriesEndogenous peroxidase (HRP) present in tissue sections can result in background when using an HRP detection system if this activity is not quenched. Methods for quenching this endogenous enzyme activity include treating with BLOXALL Endogenous Enzyme Blocking Solution or with various preparations of hydrogen peroxide.

In this video tutorial, Vector Laboratories demonstrate how to block endogenous peroxidase using a hydrogen peroxide incubation procedure.

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