Single-Domain Antibodies

Superior staining performance with Biotium MiniMab™

Our partner Biotium's highly experienced chemists and biologists are passionate about developing novel ﬂuorescent dyes and products to drive breakthroughs in life science and medical research.

Discover superior staining performance with MiniMab™ SdAbs paired with Biotium’s industry-leading CF® Dyes, outperforming conventional antibodies in immunofluorescence and beyond.

Features

Superior to Conventional Abs: Faster staining, with greater solubility, stability, and deeper tissue penetration over traditional IgG antibodies
Ideal for Super-Resolution: Smaller size leads to smaller epitope-dye displacement, ideal for STORM and other super-resolution techniques
Bright & Photostable CF® Dyes: Available conjugated to industry-leading CF® Dyes, with unique near-IR options and best-in-class dyes for STORM

What are Single-Domain Antibodies?

Single-domain antibodies (SdAbs), or VHH domains, are small antigen-binding fragments from camelid heavy chain antibodies (HCAbs) found in alpacas, llamas, and camels. Unlike conventional IgGs, which use both heavy and light chains, HCAbs (IgG2 and IgG3) lack light chains and CH1 domains, relying on a single variable domain for antigen recognition. MiniMab™ SdAbs are recombinant versions of these VHH domains.

Unlock Deeper Insights with MiniMab™ SdAbs

MiniMab™ single-domain antibodies (SdAbs) from Biotium are a next-generation labeling tool that leverage the power of SdAbs for superior performance in immunofluorescence and other applications. Their smaller ~15 kDa size relative to conventional antibodies enables deeper tissue penetration, faster staining times, and improved access to tightly packed or masked epitopes—making them ideal for high-resolution and super-resolution imaging. MiniMabs™ also offer minimal epitope-dye displacement, preserving spatial accuracy for techniques like STORM and other super-resolution methods.

Top CF® Dyes for IF & Super-Resolution

MiniMab™ SdAbs are offered conjugated to Biotium’s industry-leading CF® Dyes, known for exceptional brightness, photostability, and broad spectral range, including NIR options like CF®740. These conjugates enable extended imaging with minimal signal loss and sharp, vivid results. With MiniMab™ CF® Dye conjugates, researchers gain a powerful toolset for both routine fluorescence microscopy and cutting-edge nanoscale visualization.

Features of MiniMab™ single-domain antibodies

Advantages over IgG antibodies: deeper tissue penetration, higher solubility and stability, and faster staining
Minimal epitope-dye displacement, perfect for super-resolution imaging
Developed and optimized for immunofluorescence
Labeled with bright, photostable CF® Dyes, including near-infrared CF®740
Available with best-in-class CF® Dyes for STORM


PFA-fixed rat brain cryosection stained with CF®568 GFAP rVHH [SdAb2409.GFAP] MiniMab™ (orange; Art. N° BOT-N001-568-200UL) and NucSpot® 680/700 (magenta; Art. N° BOT-41035). Scale bar: 20 µm.	Rat retina cryosection stained with CF®488A SYT1 rVHH [SdAb2501.SYT1] MiniMab™ (green; Art. N° BOT-N002-488A-200UL), anti-GFAP CF®740 (magenta), and NucSpot® 568/580 (red; Art. N° BOT-41036).	Rat eye cryosection stained with CF®568 VGLUT1 rVHH [SdAb2412.VGLUT1] MiniMab™ (orange; Art. N° BOT-N003-568-200UL). Nuclei are stained with NucSpot® 680/700 (Art. N° BOT-41035). Scale bar: 50 µm.	MCF7 cells stained with Nuclear Membrane Antibody (2406.NM) and CF®488A Alpaca Anti-Mouse IgG1 (Fc), rVHH [N2504.M1FC] MiniMab™ (Art. N° BOT-N004-488A-500UL).