COVID-19 Mutant Proteins

Recombinant SARS-CoV-2 Spike Protein Variants

At BIOZOL, we provide you with recombinant SARS-CoV-2 spike-proteins containing the latest identified mutations, reason for the increased transmissibility of the U.K. and Brazil variant. 

Sequence and structure of the COVID-19 pathogen SARS-CoV-2 are well studied and described. Of particular interest with respect to diagnostics and drug development is the spike protein (S-protein) due to its role in the interaction with human host receptors. Since the beginning of the circulation of SARS-CoV-2 in humans, the viruses acquired an increasing number of polymorphic nucleotide positions. The influence of these mutations on properties like transmissibility, virulence or immunogenicity is object of intensive investigation. 

Viral variants harboring the D614G spike mutation were rare at the onset of the pandemic but are now prevalent worldwide, attributed to increased transmissibility (Korber et al.). Three fast-spreading new variants have emerged in the recent months: the U.K. variant (Alpha variant) B.1.1.7 (Rambaud et al.), the Brazil variant B.1.1.28 P.1 (Gamma variant), and the South Africa variant (Beta variant) B.1.351 (Tegally et al.).

A subset of the mutations identified in the RBD domain of the spike protein occurs in more than one strain, although those variants are believed to be independently evolved. These convergent mutations, specifically, the N501Y (shared between B.1.1.7, B.1.351 and P.1) and E484K (shared between B.1.351, P.1, and the Sweden-1 variant), are of high interest because they may be the cause of the increased transmissibility (Zahradnik et al.). Furthermore, the K417N and E484K polymorphisms reduce sensitivity to neutralizing antibodies (Greaney et al.), which may indicate reduced efficacy of the immune response after infection and vaccination, respectively.

Fig: 3D structure of Spike trimer embedded in lipid bilayer. 
Mutants are shown green and labelled accordingly. (Source: The Native Antigen)

 

To help you study and track the newly occuring variants of SARS-CoV-2, our suppliers make efforts to provide you with recombinant spike proteins of the various mutants the fastest possible. Several mutant proteins are already available from our partners Signalchem, Sino Biologicals and The Native Antigen.

 

Selected SARS-CoV-2 Mutant Recombinant Spike Proteins:

 

Mutation(s)	Art.-Nr.	Product
D614G (European Variant, B1)	SCM-C19S1-G231H	Signalchem SARS-CoV-2 Spike Protein S1 (D614G)
	SIN-40591-V08H3	Sino Biological SARS-CoV-2 S1 Spike Protein (D614G)-His, HPLC-verified
del69-70, del144, N501Y, A570D, D614G, P681H,T716I, S982A, D1118H (Alpha Variant, B.1.1.7)	GTX136059-PRO	SARS-CoV-2 (COVID-19) Spike (del69-70,del144,N501Y,A570D,D614G,...) (ECD) Protein, His tag (active), Unconjugated
	NAC-REC31924-100	SARS-CoV-2 (B.1.1.7) Stabilized Spike Glycoprotein, His-Strep-Tag (HEK293)
K417N, E484K, N501Y (Beta Variant, B.1.351)	GTX136095-PRO	SARS-CoV-2 (COVID-19) Spike S1 (L18F, D80A,..., R246I, K417N, E484K, N501Y, D614G), His tag (active), Unconjugated
	GTX136022-PRO	SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active), Unconjugated
	NAC-REC31963-100	SARS-CoV-2 (B.1.351) Stabilized Spike Glycoprotein (Trimeric), His-Strep-Tag (HEK293)
	BIV-P1645-50	BioVision Human CellExp(TM) SARS-CoV-2 Spike RBD (K417N, E484K, N501Y)
	ELA-PKSV030319	Recombinant SARS-CoV-2 Spike RBD(K417N,E484K,N501Y)(His Tag)
L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F (Gamma Variant, B.1.1.28 P1)	NAC-REC31944-100	SARS-CoV-2 (B.1.1.28)Stabilized Spike Glycoprotein (Full-Length), His-Strep-Tag (HEK293)
L452R, T478K (Delta Variant, B.1.617.2)	GTX136332-PRO	SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag, Unconjugated
E484Q, L452R (Kappa Variant, B.1.617.1)	NAC-REC31971-100	SARS-CoV-2 (B.1.617.1: E484Q, L452R) Spike Glycoprotein (S1) RBD, His-Tag (HEK293)
	GTX136299-PRO	SARS-CoV-2 (COVID-19) Spike RBD (L452R, E484Q Mutant) protein, His tag (active), Unconjugated
N501Y (B.1.1.7, B.1.351 and P.1)	SIN-40592-V08H82	SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein
D614G, E484K (Sweden-1)	NAC-REC31902-100	SARS-CoV-2 (D614G, E484K Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)
D614G, S477N (England-1)	NAC-REC31899-100	SARS-CoV-2 (D614G, S477N Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)
D614G, L84I, N439K (Scotland-1)	NAC-REC31900-100	SARS-CoV-2 (D614G, L84I, N439K Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)
D614G, G485R (Australia-1)	NAC-REC31901-100	SARS-CoV-2 (D614G, G485R Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)
D614G, V445I, H655Y, E583D (England/Bristol-1)	NAC-REC31903-100	SARS-CoV-2 (D614G, V445I, H655Y, E583D Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)
Y453F (B1.1.298)	SIN-40592-V08H80	SARS-CoV-2 Spike RBD(Y453F)-His Recombinant Protein
N439K (B.1.258, B.1.141 / B1.258)	SIN-40592-V08H14	SARS-CoV-2 (2019-nCoV) Spike RBD(N439K)-His Recombinant Protein

 

From System Biosciences, Lentivector Packaging Mixes are available, pseudotyped with the SARS-CoV-2 S1 spike protein in several variants, including the Southafrican variant B.1.351 (K417N, E484K, N501Y, and D614G).

Further SARS-CoV-2 mutant spike proteins are in development at several of our suppliers and will soon be available. If you are looking for a specific mutation, which is not yet available in our web store, please feel free to contact us and we will do our best to find the right protein for you.