COVID-19 Mutant Proteins
Recombinant SARS-CoV-2 Spike Protein Variants
At BIOZOL, we provide you with recombinant SARS-CoV-2 spike-proteins containing the latest identified mutations, reason for the increased transmissibility of the U.K. and Brazil variant.
Sequence and structure of the COVID-19 pathogen SARS-CoV-2 are well studied and described. Of particular interest with respect to diagnostics and drug development is the spike protein (S-protein) due to its role in the interaction with human host receptors. Since the beginning of the circulation of SARS-CoV-2 in humans, the viruses acquired an increasing number of polymorphic nucleotide positions. The influence of these mutations on properties like transmissibility, virulence or immunogenicity is object of intensive investigation.
Viral variants harboring the D614G spike mutation were rare at the onset of the pandemic but are now prevalent worldwide, attributed to increased transmissibility (Korber et al.). Three fast-spreading new variants have emerged in the recent months: the U.K. variant B.1.1.7 (Rambaud et al.), the Brazil variant B.1.1.28 P.1, and the South Africa variant B.1.351 (Tegally et al.).
A subset of the mutations identified in the RBD domain of the spike protein occurs in more than one strain, although those variants are believed to be independently evolved. These convergent mutations, specifically, the N501Y (shared between B.1.1.7, B.1.351 and P.1) and E484K (shared between B.1.351, P.1, and the Sweden-1 variant), are of high interest because they may be the cause of the increased transmissibility (Zahradnik et al.). Furthermore, the K417N and E484K polymorphisms reduce sensitivity to neutralizing antibodies (Greaney et al.), which may indicate reduced efficacy of the immune response after infection and vaccination, respectively.
Fig: 3D structure of Spike trimer embedded in lipid bilayer.
Mutants are shown green and labelled accordingly. (Source: The Native Antigen)
To help you study and track the newly occuring variants of SARS-CoV-2, our suppliers make efforts to provide you with recombinant spike proteins of the various mutants the fastest possible. Several mutant proteins are already available from our partners Signalchem, Sino Biologicals and The Native Antigen.
Selected SARS-CoV-2 Mutant Recombinant Spike Proteins:
(European Variant, B1)
|SCM-C19S1-G231H||Signalchem SARS-CoV-2 Spike Protein S1 (D614G)|
Sino Biological SARS-CoV-2 S1 Spike Protein (D614G)-His, HPLC-verified
(B.1.1.7, B.1.351 and P.1)
|SIN-40592-V08H82||SARS-CoV-2 (2019-nCoV) Spike RBD(N501Y)-His Recombinant Protein|
|NAC-REC31902-100||SARS-CoV-2 (D614G, E484K Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)|
|NAC-REC31899-100||SARS-CoV-2 (D614G, S477N Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)|
D614G, L84I, N439K
|NAC-REC31900-100||SARS-CoV-2 (D614G, L84I, N439K Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)|
|NAC-REC31901-100||SARS-CoV-2 (D614G, G485R Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)|
D614G, V445I, H655Y, E583D
|NAC-REC31903-100||SARS-CoV-2 (D614G, V445I, H655Y, E583D Mutant) Spike Glycoprotein (S1), Sheep Fc-Tag (HEK293)|
|SIN-40592-V08H80||SARS-CoV-2 Spike RBD(Y453F)-His Recombinant Protein|
(B.1.258, B.1.141 / B1.258)
|SIN-40592-V08H14||SARS-CoV-2 (2019-nCoV) Spike RBD(N439K)-His Recombinant Protein|
Further SARS-CoV-2 mutant spike proteins are in development at several of our suppliers and will soon be available. If you are looking for a specific mutation, which is not yet available in our web store, please feel free to contact us and we will do our best to find the right protein for you.