Afamin/Wnt3a Complex

Serum-free Long-term Organoid Culture

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Afamin/Wnt3a CM (High Concentration)

 

• Stable Wnt3a activity
• Enables organoid culture with serum-free medium
• Enables long-term organoid culture
• New product variant: Higher density and higher activity

 

Mechanism of Wnt3a stabilization by Afamin:

Wnt3a is a common growth factor used in organoid culture. However, the lipid-soluble form of the Wnt3a protein tends to aggregate in serum-free medium over time, which leads to a loss of activity.

Mihara et al. discovered that Afamin, a serum component, forms a complex with Wnt3a, thereby maintaining its activity. Furthermore, using the Afamin-Wnt3a complex enabled long-term organoid culture.

Intestinal organoid culture using Afamin/Wnt3a CM

Both the new product (art. no. MBL-J2-005-05) and the previous product (art. no. MBL-J2-001) were used to culture human small intestine organoids. At a concentration of 2.5%, the former product was found to decrease organoid growth (see bottom image). Even at an added concentration of 2.5%, the new product maintains robust organoid growth (top).

 

Afamin/Wnt3a CM (High Concentration)

Art. N°	Product Name	Main Components	Solvent	Size
MBL-J2-005-05	Afamin/Wnt3a CM (High Concentration)	Mouse Wnt3a Human Afamin	Expi293TM Expression Medium	5 mL

 

Related Products

Art. N°	Product Name	Main Components	Solvent	Size
MBL-J2-001	Afamin/Wnt3a CM	Mouse Wnt3a Human Afamin	Advanced D-MEM/F-12	10 mL
MBL-J2-002	Recombinant Afamin/Wnt3a CM	Mouse Wnt3a Human Afamin	20 mM Tris-HCl (pH7.4), 150 mM NaCl	60 µg / 300 µl