UPF2 Rabbit pAb, Unconjugated

Product Overview

• Article Name: UPF2 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A13411
• Supplier Catalog Number: A13411
• Alternative Catalog Number: ABB-A13411-100UL,ABB-A13411-20UL,ABB-A13411-1000UL,ABB-A13411-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HUPF2, RENT2, smg-3, UPF2

Product Description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene, both variants encode the same protein.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 148kDa
• NCBI: 26019
• UniProt: Q9HAU5
• Purity: Affinity purification
• Sequence: GPPLGGGEGEAESADTMPFVMLTRKGNKQQFKILNVPMSSQLAANHWNQQQAEQEERMRMKKLTLDINERQEQEDYQEMLQSLAQRPAPANTNRERRPRYQHPKGAPNADLIFKTGGRRR
• Target: UPF2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|IHC-P,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Rat, ResearchArea: Epigenetics Nuclear Signaling.

Images

Immunohistochemistry analysis of paraffin-embedded Rat kidney using UPF2 Rabbit pAb (A13411) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug UPF2 Rabbit pAb (A13411 1:200). Western blot was performed from the immunoprecipitate using UPF2 Rabbit pAb (A7091) at a dilition of 1:1000.

Western blot analysis of various lysates, using UPF2 Rabbit pAb (A13411) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.