PGP9.5/UCHL1 Rabbit mAb, Unconjugated

Product Overview

• Article Name: PGP9.5/UCHL1 Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A20380
• Supplier Catalog Number: A20380
• Alternative Catalog Number: ABB-A20380-100UL, ABB-A20380-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: NDGOA, PARK5, PGP95, SPG79, PGP9.5, SPG79A, UCHL-1, Uch-L1, HEL-117, PGP 9.5, HEL-S-53, PGP9.5/UCHL1

Product Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.

Product Properties

• Molecular Weight: 25kDa
• NCBI: 7345
• UniProt: P09936
• Purity: Affinity purification
• Target: UCHL1

Application Notes

• Application Dilute: WB,1:10000 - 1:50000|IF/ICC,1:500 - 1:1000|IF-P,1:500 - 1:1000|IHC-P,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Cell Biology Developmental Biology,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Neuroscience, Cell Type Marker,Neurodegenerative Diseases,Dopamine Signaling in Parkinsons Disease,Neuron marker.

Images

Western blot analysis of various lysates, using PGP9.5/UCHL1 Rabbit mAb (A20380) at 1:47000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat pancreas tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Confocal imaging of C6 cells using PGP9.5/UCHL1 Rabbit mAb (A20380,dilution 1:1000)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Immunofluorescence analysis of Mouse brain tissue using PGP9.5/UCHL1 Rabbit mAb (A20380) at a dilution of 1:1000 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.