[KD Validated] LAMP1/CD107a Rabbit mAb, Unconjugated

Product Overview

• Article Name: [KD Validated] LAMP1/CD107a Rabbit mAb, Unconjugated
• Biozol Catalog Number: ABB-A21194
• Supplier Catalog Number: A21194
• Alternative Catalog Number: ABB-A21194-100UL,ABB-A21194-20UL,ABB-A21194-500UL,ABB-A21194-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FC, IF, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: LAMPA, CD107a, LGP120

Product Description

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [ARC52154]
• Molecular Weight: 38kDa/45kDa
• NCBI: 3916
• UniProt: P11279
• Purity: Affinity purification
• Sequence: AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGT
• Target: LAMP1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:10000 - 1:40000|IHC-P,1:500 - 1:5000|IF/ICC,1:200 - 1:2000|FC (intra),1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Cancer,Signal Transduction,Cell Biology Developmental Biology,Autophagy,Endocrine Metabolism,Immunology Inflammation,CDs,Neuroscience, Cell Type Marker,Stem Cells,Hematopoietic Progenitors,Neuron marker.

Images

Western blot analysis of lysates from wild type (WT) and LAMP1 knockdown (KD) HeLa cells, using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Western blot analysis of lysates from U-251MG cells, using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded HeLa and HeLa-LAMP1-KO cells using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194) at a dilution of 1:2200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Confocal imaging of HeLa and HeLa-LAMP1-KD cells using [KD Validated] LAMP1/CD107a Rabbit mAb (A21194,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow cytometry: 1X10 6 knockdown (KD) HeLa cells (negative control,left)and HeLa cells (right) were intracellularly-stained with [KD Validated] LAMP1/CD107a Rabbit mAb (A21194,2.5 µg/mL,orange line) or ABflo 488 Rabbit IgG isotype control (A22069,5 µl/Test,blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10 6 HeLa cells were intracellularly-stained with ABflo 488 Rabbit IgG isotype control (A22069,5 µl/Test,left) or [KD Validated] LAMP1/CD107a Rabbit mAb (A21194,2.5 µg/mL,right).