KAP1/TRIM28 Rabbit pAb, Unconjugated

Product Overview

• Article Name: KAP1/TRIM28 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-A2245
• Supplier Catalog Number: A2245
• Alternative Catalog Number: ABB-A2245-100UL, ABB-A2245-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC-P, IP, WB
• Species Reactivity: Human
• Immunogen: This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: KAP1, TF1B, RNF96, TIF1B, PPP1R157, TIF1beta, KAP1/TRIM28

Product Description

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Product Properties

• Molecular Weight: 89kDa
• NCBI: 10155
• UniProt: Q13263
• Purity: Affinity purification
• Target: TRIM28

Application Notes

• Application Dilute: WB,1:1000 - 1:5000|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|IF/ICC,1:50 - 1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Invasion and Metastasis,Cell Biology Developmental Biology,Apoptosis,Ubiquitin.

Images

Western blot analysis of various lysates using KAP1/TRIM28 Rabbit pAb (A2245) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using KAP1/KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using KAP1/KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using KAP1/KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat ovary using KAP1/KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using KAP1/TRIM28 Rabbit pAb (A2245) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KAP1/TRIM28 Rabbit pAb (A2245 1:400). Western blot was performed from the immunoprecipitate using KAP1/TRIM28 Rabbit pAb (A2245) at a dilition of 1:1000.