NKX2-2 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A25248
Article Name: NKX2-2 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A25248
Supplier Catalog Number: A25248
Alternative Catalog Number: ABB-A25248-20UL,ABB-A25248-100UL,ABB-A25248-1000UL,ABB-A25248-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: NKX2B, NKX2.2, NKX2-2
The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
Clonality: Polyclonal
Molecular Weight: 30kDa
NCBI: 4821
UniProt: O95096
Purity: Affinity purification
Sequence: KSPEPSADESPDNDKETPGGGGDAGKKRKRRVLFSKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPR
Target: NKX2-2
Antibody Type: Primary Antibody
Application Dilute: WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cell Biology Developmental Biology,Neuroscience,Stem Cells. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and293T cells transfected withNKX2-2 usingNKX2-2 Rabbit pAb (A25248) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:1s.