Estrogen Receptor beta Rabbit pAb, Unconjugated, Polyclonal

Product Overview

• Article Name: Estrogen Receptor beta Rabbit pAb, Unconjugated, Polyclonal
• Biozol Catalog Number: ABB-A2546
• Supplier Catalog Number: A2546
• Alternative Catalog Number: ABB-A2546-100UL,ABB-A2546-20UL,ABB-A2546-500UL,ABB-A2546-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: Erb, ESRB, ODG8, ESTRB, NR3A2, ER-BETA, ESR-BETA, Estrogen Receptor beta

Product Description

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 59kDa
• NCBI: 2100
• UniProt: Q92731
• Purity: Affinity purification
• Sequence: MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCS
• Target: ESR2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Nuclear Receptor Signaling,Nuclear hormone receptors,Cancer,Tumor biomarkers,Signal Transduction,MAPK-Erk Signaling Pathway,Endocrine Metabolism,Endocrine and metabolic diseases,Diabetes,Obesity,Neuroscience. Shipping: Ice Bag

Images

Western blot analysis of lysates from Daudi cells usingEstrogen Receptor beta Rabbit pAb (A2546) at1:600 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.