Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb, Unconjugated, Monoclonal

Product Overview

• Article Name: Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb, Unconjugated, Monoclonal
• Biozol Catalog Number: ABB-A3730
• Supplier Catalog Number: A3730
• Alternative Catalog Number: ABB-A3730-100UL,ABB-A3730-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1, Prolyl hydroxylase PHD1 (EGLN2)

Product Description

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Product Properties

• Clonality: Monoclonal
• Molecular Weight: 44kDa
• NCBI: 112398
• UniProt: Q96KS0
• Purity: Affinity purification
• Target: EGLN2

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Cancer,Invasion and Metastasis,Endocrine Metabolism,Cardiovascular,Hypoxia. Shipping: Ice Bag

Images

Western blot analysis of various lysates using Prolyl hydroxylase PHD1 (EGLN2) Rabbit mAb (A3730) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.