Phospho-MDM2-S260 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-MDM2-S260 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0074
• Supplier Catalog Number: AP0074
• Alternative Catalog Number: ABB-AP0074-100UL, ABB-AP0074-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HDMX, LSKB, hdm2, ACTFS, Phospho-MDM2-S260

Product Description

This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

Product Properties

• Molecular Weight: 55kDa
• NCBI: 4193
• UniProt: Q00987
• Purity: Affinity purification
• Target: MDM2

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,PI3K-Akt Signaling Pathway,ErbB-HER Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G2 M DNA Damage Checkpoint,Ubiquitin,Ubiquitin-Proteasome Signaling Pathway,Immunology Inflammation,Neuroscience.

Images

Western blot analysis of lysates from 293 cells, using Phospho-MDM2-S260 Rabbit pAb (AP0074).
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.