Phospho-HDAC5-S498 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-HDAC5-S498 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0202
• Supplier Catalog Number: AP0202
• Alternative Catalog Number: ABB-AP0202-100UL, ABB-AP0202-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: HD5, NY-CO-9, Phospho-HDAC5-S498

Product Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

Product Properties

• Molecular Weight: 122kDa
• NCBI: 10014
• UniProt: Q9UQL6
• Purity: Affinity purification
• Target: HDAC5

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics Nuclear Signaling,Nuclear Receptor Signaling,Protein phosphorylation,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Cell Cycle Control-G1 S Checkpoint,Wnt -Catenin Signaling Pathway,Immunology Inflammation,NF-kB Signaling Pathway,Stem Cells,Cardiovascular,Heart,Hypertrophy.

Images

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HDAC5-S498 Rabbit pAb (AP0202) at 1:2000 dilution. NIH/3T3 cells were treated with Insulin (100nM) for 10 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.