Phospho-PRMT4/CARM1-S228 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PRMT4/CARM1-S228 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0319
• Supplier Catalog Number: AP0319
• Alternative Catalog Number: ABB-AP0319-20UL,ABB-AP0319-1000UL,ABB-AP0319-100UL,ABB-AP0319-500UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: PRMT4, Phospho-PRMT4/CARM1-S228

Product Description

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 66kDa
• NCBI: 10498
• UniProt: Q86X55
• Purity: Affinity purification
• Sequence: VKSNN
• Target: CARM1
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Nuclear Receptor Signaling,Nuclear hormone receptors,Cancer,Tumor suppressors,p53 pathway,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Endocrine Metabolism,Lipid Metabolism.

Images

Western blot analysis of lysates from Jurkat cells, using Phospho-PRMT4/CARM1-S228 Rabbit pAb (AP0319) at 1:1000 dilution. Jurkat cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.