Phospho-ALK-Y1096 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-ALK-Y1096 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0503
• Supplier Catalog Number: AP0503
• Alternative Catalog Number: ABB-AP0503-500UL,ABB-AP0503-100UL,ABB-AP0503-20UL,ABB-AP0503-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: ALK1, CD246, NBLST3, Phospho-ALK-Y1096

Product Description

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 176kDa
• NCBI: 238
• UniProt: Q9UM73
• Purity: Affinity purification
• Sequence: PNYCF
• Target: ALK
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Growth factors,Immunology Inflammation,CDs,Neuroscience, Cell Type Marker,Neuron marker.

Images

Western blot analysis of lysates from HL-60 and HL-60 cells, using Phospho-ALK-Y1096 Rabbit pAb (AP0503) at 1:1000 dilution. HL-60 cells were treated with 10% FBS after serum-starvation overnight. HL-60 cells were treated with UV for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.