Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0785
• Supplier Catalog Number: AP0785
• Alternative Catalog Number: ABB-AP0785-20UL,ABB-AP0785-100UL,ABB-AP0785-500UL,ABB-AP0785-1000UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: FCAS3, APLAID, PLC-IV, PLC-gamma-2, Phospho-PLC gamma 2 (PLCG2)-Y759

Product Description

The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Product Properties

• Clonality: Polyclonal
• Molecular Weight: 148kDa
• NCBI: 5336
• UniProt: P16885
• Purity: Affinity purification
• Sequence: RMYVD
• Target: PLCG2
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,Endocrine Metabolism,Lipid Metabolism,Immunology Inflammation,B Cell Receptor Signaling Pathway,Cardiovascular,Angiogenesis,Lipids.

Images

Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y759 Rabbit pAb (AP0785) at 1:2000 dilution. Raji cells were treated with Pervanadate (1 mM) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.