Phospho-DRP1-S616 Rabbit pAb, Unconjugated

Product Overview

• Article Name: Phospho-DRP1-S616 Rabbit pAb, Unconjugated
• Biozol Catalog Number: ABB-AP0849
• Supplier Catalog Number: AP0849
• Alternative Catalog Number: ABB-AP0849-1000UL,ABB-AP0849-500UL,ABB-AP0849-20UL,ABB-AP0849-100UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Conjugation: Unconjugated
• Alternative Names: DLP1, DRP1, DVLP, EMPF, OPA5, EMPF1, DYMPLE, HDYNIV, Phospho-DRP1-S616

Product Description

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimers disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Properties

• Molecular Weight: 82 kDa
• NCBI: 10059
• UniProt: O00429
• Purity: Affinity purification
• Sequence: PASPQ
• Target: DNM1L

Application Notes

• Application Dilute: WB,1:500 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Neuroscience,Neurodegenerative Diseases,Dopamine Signaling in Parkinsons Disease.

Images

Western blot analysis of lysates from HeLa cells, using Phospho-DRP1-S616 Rabbit pAb (AP0849) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.