Phospho-Ezrin-Y353 Rabbit pAb

Product Overview

• Article Name: Phospho-Ezrin-Y353 Rabbit pAb
• Biozol Catalog Number: ABB-AP1118
• Supplier Catalog Number: AP1118
• Alternative Catalog Number: ABB-AP1118-100UL, ABB-AP1118-20UL
• Manufacturer: ABclonal
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Alternative Names: CVL, CVIL, VIL2, HEL-S-105, Phospho-Ezrin-Y353

Product Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Product Properties

• Molecular Weight: 69kDa
• NCBI: 7430
• UniProt: P15311
• Purity: Affinity purification
• Target: EZR

Application Notes

• Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Application Notes: Cross-Reactivity: Human, ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Protein phosphorylation,Cancer,Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Microfilaments,Immunology Inflammation,B Cell Receptor Signaling Pathway.

Images

Western blot analysis of lysates from 293T cells, using Phospho-Ezrin-Y353 Rabbit pAb (A0703). 293T cells were treated with ATP(5 mM) at 30°C for 1 hour.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.